发明名称 |
SINGLE MOLECULE SEQUENCING METHOD, DEVICE, SYSTEM, AND APPLICATION |
摘要 |
The present invention provides a single molecule sequencing (SMTS) method, device, system, and application, and the method comprising: (i) combining a template nucleic acid having a first optical detection marker at an end with a primer to obtain a first complex; (ii) imaging the first complex to obtain a first image; (iii) mixing the first complex, a polymerase, and at least one nucleotide having an optical detection marker, and obtaining an extended product by a polymerase reaction; (iv) imaging the extended first complex to obtain a second image; (v) removing a breakable group from the nucleotide having the optical detection marker of the extended product to obtain a second complex; and (vi) repeating step (ii) to (v) one or more times to identify a sequence of the template nucleic acid. The single molecule sequencing method provided by the present invention achieves single molecule sequencing via a primer capturing a template nucleic acid. |
申请公布号 |
WO2017028760(A1) |
申请公布日期 |
2017.02.23 |
申请号 |
WO2016CN95053 |
申请日期 |
2016.08.12 |
申请人 |
DIRECT GENOMICS CO., LTD. |
发明人 |
GE, Liangjin;GAO, Yan;DENG, Liyu;WU, Zengding;CAI, Jinsen;JI, Daorui;ZENG, Lidong;LI, Gailing |
分类号 |
C12Q1/68;C12M1/34;C12M1/36;C12M1/38 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|