| 摘要 |
A single cell control method based on a light-induced dielectrophoresis technique, comprising the steps: A. fabricating a light-induced dielectrophoresis chip (20), wherein the light-induced dielectrophoresis chip (20) is composed of a three-layer structure: the lower layer being ITO glass coated with a hydrogenated amorphous silicon coating layer, the upper layer being ITO glass without a coating layer, and a micro-fluidic channel being encapsulated between the upper and lower ITO glass layers for injecting a solution required for operations; B. inputting a variable frequency alternating current signal to electrodes of the upper and lower ITO glass layers, and at the same time using incident light to irradiate the light-induced dielectrophoresis chip (20), so as to generate a non-uniform electric field in an irradiated area; and C. under the real-time observation of a microscope image system, realizing cell control by changing the frequency and amplitude of the alternating current signal. The control method has the advantages of low cost, simple operation and high efficiency. |
