发明名称 |
METHODS FOR ANALYZING THE INTERACTION BETWEEN A TARGET PROTEIN AND A LIGAND |
摘要 |
Provided is a method for simultaneously determining both [L]0 and Kd values of a ligand for a target protein. In one embodiment, the present technology involves performing quantitative equilibrium immunoassays at two different concentrations of the target and fitting the data to simultaneously determine Kd and [L]0. Also provided is a method for determining binding affinity of a pool of candidate ligands in a high-throughput manner. In another embodiment, the present technology method combines high-throughput nucleic acid sequencing with a display technology to obtain kinetic on-rates and off-rates, and thus Kd values, for the candidate ligands. |
申请公布号 |
WO2016210009(A3) |
申请公布日期 |
2017.02.23 |
申请号 |
WO2016US38830 |
申请日期 |
2016.06.22 |
申请人 |
UNIVERSITY OF SOUTHERN CALIFORNIA |
发明人 |
ROBERTS, Richard;JALALI-YAZDI, Farzad |
分类号 |
G01N33/53;G01N33/557;G01N33/563;G01N33/566;G01N33/577;G01N33/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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