METHODS AND APPARATUS FOR TRANSFORMATION OF NATURALLY COMPETENT CELLS

摘要

The present invention includes compositions and methods of co-transformation of naturally competent cells. In one aspect of the invention, a method is included for introducing nucleic acid sequences into one or more naturally competent cells in parallel. In other aspects, a heterogenic pool of co-transformed naturally competent cells and an apparatus for introducing two or more populations of nucleic acid sequences into a population of naturally competent cells in parallel are also included.

主权项

1. A method of introducing nucleic acid molecules into one or more cells in parallel comprising:
(a) contacting naturally competent cells with two or more nucleic acid molecules, wherein at least one of the nucleic acid sequences comprises a selectable marker; and (b) selecting for that marker.