| 发明名称 |
CHROMATOGRAPHY METHOD |
| 摘要 |
The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof. |
| 申请公布号 |
US2017051010(A1) |
申请公布日期 |
2017.02.23 |
| 申请号 |
US201515308327 |
申请日期 |
2015.06.04 |
| 申请人 |
GE Healthcare BioProcess R&D AB |
发明人 |
Maloisel Jean-Luc;Lind Ola;Noren Bjorn;Palmgren Ronnie |
| 分类号 |
C07K1/22;C07K16/00 |
主分类号 |
C07K1/22 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for purification of immunoglobulin containing proteins or parts thereof, from larger contaminants, comprising: a) contacting an immunoglobulin containing sample with chromatography beads comprising a thin lid with a defined pore size and an inner core of defined pore size provided with an affinity ligand; b) adsorbing the immunoglobulin containing proteins to the ligands; c) washing the beads to remove any contaminants; and d) eluting the beads to release the captured immunoglobulin containing proteins or parts thereof. |
| 地址 |
Uppsala SE |
