SMALL ncRNAS AS BIOMARKERS

摘要

Disclosed are small ncRNAs that may be used as biomarkers for classifying the health status of an individual. The disclosure also provides screening methods for identifying ncRNA biomarkers.

主权项

1. A method for identifying a small non-coding RNA (ncRNA) biomarker pair, the method comprising
determining the quantity of two different varieties of said ncRNA in a first bodily fluid sample obtained from a first individual reference and in a second bodily fluid sample obtained from a second individual reference, wherein said first individual reference has an altered health status from said second individual reference, determining the ratio of the two different varieties in the first bodily fluid sample, determining the ratio of the two different varieties in the second bodily fluid sample, comparing the ratios from said first and second bodily fluid sample, and identifying the two different varieties of said ncRNA as a biomarker pair when the ratio is altered between said first and second bodily fluid sample, wherein the first and second varieties are selected from the canonical ncRNA; the ncRNA trimmed at the 5′ or 3′ end and/or extended at the 5′ or 3′ end; and the ncRNA with a 3′ non-templated nucleotide addition, optionally trimmed at the 5′ or 3′ end or extended at the 5′ or 3′ end; wherein when said ncRNA is not an miRNA the first variety is selected from the canonical ncRNA and the ncRNA with a 3′ non-templated nucleotide addition and the second variety is the ncRNA with a 3′ non-templated nucleotide addition.