Temperature Gradient Surface Plasmon Resonance Instrument

摘要

A system and method for sensitive measurements of molecular binding as a function of time and temperature provided by a surface plasmon resonance (SPR) instrument producing a stable temperature gradient over the sensor surface. Continuous monitoring of reflected light intensity from different locations of the sensor allows simultaneous measurement of time and temperature dependence of binding interactions.

主权项

1. An apparatus for evaluating interactions between biological oligomers comprising:
a light source; a camera; an optical coupler positioned between the light source and the camera to receive light from the light source for reflection from an active face of the optical coupler to be received by the camera; a metal film extending along a measurement plane proximate to the active face for holding oligomers subject to interaction on a first face of the metal film; a flow channel opening against the first face of the metal film for receiving a liquid flow in a direction therealong; and a first and second thermal stabilizer positioned in thermal communication with the metal film and separated along the measurement plane near opposite edges of the metal film and adapted to establish a predetermined and substantially constant temperature gradient along the metal film.