SEQUENCE IMMOBILIZATION METHOD, CHIP, AND APPLICATION

摘要

The present invention provides a sequence immobilization method, chip, and application, the method comprising: (1) immersing a substrate in an immobilization solution of 0.4-3.2 nM containing a sequence to obtain a sequence-carrying substrate, wherein the substrate is a solid matrix having a chemically-modified surface, an end of the sequence has a 10-30 bp polyT, and the immobilization solution is a phosphate buffer; and (2) performing passivation by immersing the sequence-carrying substrate in a passivation solution to obtain a passivated sequence-carrying substrate, wherein the passivation solution is a phosphate solution. The sequence immobilization provided by the present invention has a uniform density, about 3000 points per visual field.