HIGHLY MULTIPLEX PCR METHODS AND COMPOSITIONS

摘要

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

主权项

1. A method for determining a ploidy status of a chromosome or chromosome segment of a cancer in a host, the method comprising:
measuring the DNA in a mixed sample that comprises DNA from normal cells from the host and DNA from the cancer at a plurality of at least 10 polymorphic loci on the chromosome or chromosome segment; determining allele counts at the plurality of polymorphic loci from the DNA measurements made on the sample; fitting, on a computer, the determined allele counts to expected allele counts at the plurality of polymorphic loci for each of a plurality of ploidy hypotheses each specifying a different possible ploidy state of the chromosome or chromosome segment for the cancer, using a joint distribution model that takes into account the expected linkage between the plurality of polymorphic loci on the chromosome or chromosome segment, to determine a relative probability of each of the ploidy hypotheses; and outputting the hypothesis with the greatest probability of being true as an indication of the ploidy status of the chromosome or chromosome segment of the cancer.