METHOD FOR ASSESSING STATE OF DIFFERENTIATION OF CELLS

摘要

In the present invention, for test cells which are either stem cells whose state of differentiation is unknown or cells obtained from stem cells by differentiation induction, an LC-MS or GC-MS analysis is performed on culture supernatants collected from a culture dish of the test cells and a culture dish of control cells whose state of differentiation is known, and the state of differentiation of the test cells is assessed based on the amount, determined as a result of the aforementioned analysis, of at least one compound selected from the group of putrescine, kynurenine, cystathionine, ascorbic acid, riboflavin, pyruvic acid, serine, cysteine, threonic acid, citric acid, and orotic acid in both the culture supernatant of the test cells and the culture supernatant of the control cells.

主权项

1. A cell differentiation state assessment method, comprising:
assessing a state of differentiation of test cells based on an amount of a specified substance in a culture supernatant of the test cells, the test cells being stem cells whose state of differentiation is unknown or cells obtained from stem cells by differentiation induction, wherein the specified substance is at least one compound selected from the group consisting of putrescine, kynurenine, cystathionine, ascorbic acid, riboflavin, pyruvic acid, serine, cysteine, threonic acid, citric acid, and orotic acid.