STRONG SECRETORY SIGNAL PEPTIDE ENHANCING SMALL PEPTIDE MOTIF AND USE THEREOF

摘要

The present invention belongs to protein engineering and genetic engineering fields, relating to a strong secretory signal peptide enhancing small peptide motifs and the use thereof. The strong secretory signal peptide enhancing small peptide motifs of the present invention have the amino acid sequence of the following formula: M (±X²Y³/±Y²X³) n , wherein X represents an acidic amino acid; Y represents an alkaline amino acid; ± is 0 to 2 neutral amino acid(s); ² represents 0 to 2 neutral amino acid(s); ³ represents 1 to 10 neutral amino acid(s); n is 1 to 3. With regard to the use of the strong secretory signal peptide enhancing small peptide motifs of the present invention, it is a method for constructing a vector enhancing the secretion ability of common signal peptides to improve the secretory expression of exogenous proteins.