TRANSPOSITION INTO NATIVE CHROMATIN FOR PERSONAL EPIGENOMICS
摘要
Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagged fragments to produce a plurality of sequence reads; and (c) making an epigenetic map of a region of the genome of the cells by mapping information obtained from the sequence reads to the region. A kit for performing the method is also provided.
申请公布号
EP2999792(A4)
申请公布日期
2017.02.22
申请号
EP20140800805
申请日期
2014.05.20
申请人
The Board of Trustees of The Leland Stanford Junior University
发明人
GIRESI, Paul;BUENROSTRO, Jason D.;CHANG, Howard Y.;GREENLEAF, William J.