Isolation of RNA-protein complexes using cross-linking reagents and oligonucleotides

摘要

Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the oligonucleotide to the cross-linked protein to produce a second cross-linked product; d) isolating the second cross-linked product using an affinity tag; and e) analyzing the isolated second cross-linked product. Compounds for performing the method are also provided.

主权项

1. A kit comprising a first compound of formula I and a second compound of formula II: wherein:
Z1 and Z2 are protein reactive groups; W and Y are conditionally reactive groups that, upon activation, specifically react with one another to covalently link B1 and P;
B1 is a first linking moiety that joins Z1, Z2 and W;A is an affinity tag; andP is an oligonucleotide that specifically hybridizes to an RNA of a cell.