Method for detecting a target particle

摘要

Provided is a method for detecting a target particle that is a method for detecting a non-luminescent target particle dispersed and randomly moving in a sample solution using an optical system of a confocal microscope or multi-photon microscope, having: (a) preparing a sample solution containing target particles, and labeling particles of which the average outer diameter is less than 15% of the diameter of a photodetection region of the optical system, binding two or more molecules of the labeling particles per molecule of the target particles in the sample solution, and forming a non-luminescent complex of which the outer diameter is 15% or more of the diameter of the photodetection region; and, (b) calculating the number of molecules of the complex in the sample solution prepared in the (a) using an inverse scanning molecule counting method.

主权项

1. A method for detecting a target particle dispersed and randomly moving in a sample solution using an optical system of a confocal microscope or multi-photon microscope, comprising:
(a) preparing a sample solution containing target particles, and labeling particles of which an average outer diameter is less than 15% of a diameter of a photodetection region of the optical system, and forming a non-luminescent complex of which an outer diameter is 15% or more of the diameter of the photodetection region by binding two or more molecules of the labeling particles to one molecule of the target particles in the sample solution; and, (b) calculating number of molecules of the complex in the sample solution prepared in step (a), comprising: moving a location of the photodetection region of the optical system in the sample solution, generating chronological light intensity data by detecting light containing substantially constant background light from the photodetection region while moving the location of the photodetection region of the optical system in the sample solution, individually detecting a decrease in light intensity in the chronological light intensity data that occurs when the complex has entered the photodetection region as a signal representing the presence of an individual complex, and counting number of the complexes detected during movement of the location of the photodetection region by counting the number of signals representing the presence of the individually detected complexes.