Determining conditions in centrifuged blood using measured pressure

摘要

A method for determining a condition in a blood sample includes: providing a sample of blood; providing a metering probe having a pump for aspirating and dispensing; inserting the metering probe a selected distance into the blood sample; measuring the pressure between the sample and pump during sample aspiration or sample dispense; comparing the measured pressure with a reference value; and signaling the presence or absence of the condition. A method for confirming or detecting the presence of a selected layer of blood component in a centrifuged blood sample includes: measuring a pressure of a suspected selected layer in a metering probe during aspiration or dispense; comparing the measured pressure with a reference value, wherein if the measured pressure and the reference value are substantially identical then the selected layer of the blood component is confirmed. In a preferred embodiment the reference value is a pre-selected pressure range.

主权项

1. A method of determining a condition in a centrifuged blood sample, comprising:
providing a sample of centrifuged blood separated into a plasma portion at the top of the centrifuged sample and a packed red blood cells portion at the bottom of the centrifuged sample; providing a metering probe having a pump for aspirating and dispensing; inserting the metering probe a selected distance into the blood sample to the bottom of the centrifuged sample until the metering probe contacts the packed red blood cells portion; measuring the pressure between the sample and pump during sample aspiration or sample dispense and generating a first pressure profile; comparing at least a portion of the first pressure profile with a reference value relating to a second pressure profile; and signaling the presence or absence of the condition, wherein the condition is an error condition caused by an incomplete centrifugation when the measured pressure is outside of the reference value.