Cell culture apparatus and cell culture method using the same

摘要

Disclosed herein is a cell culture apparatus that can achieve appropriate culture conditions. The cell culture apparatus (1) includes: a cylindrical culture vessel (10) that holds a culture liquid containing cells; a supporting column (20) that stands upright in a center of an inner surface of a bottom (12) in the culture vessel; and a stirring device (30) that includes an attaching portion (32) that is attached to an upper portion of the supporting column so as to be rotatable relative to the supporting column and a stirring blade (34) whose upper portion is fixed to the attaching portion so as to rotate around the supporting column.

主权项

1. A cell culture apparatus comprising:
a cylindrical culture vessel that holds a culture liquid containing cells; a supporting column that is fixed to stand upright in a center of an inner surface of a bottom of the culture vessel; and a stirring device comprising an attaching portion that is attached to an upper portion of the supporting column so as to be rotatable relative to the supporting column and a stirring blade, an upper portion of the stirring blade being fixed to the attaching portion so as to rotate around the supporting column as a center of rotation, the stirring blade of the stirring device having a plate shape extending downwards, as a whole, from the upper portion of the stirring blade, wherein the supporting column has a conical portion, the conical portion having a portion conically formed to rise up from the inner surface of the bottom and the conical portion having a diameter increasing toward the inner surface of the bottom, the upper portion of the supporting column is located above a liquid level of the culture liquid, and the stirring blade of the stirring device is formed so that a lower portion of the stirring blade has a shape that conforms to an outer surface of the conical portion of the supporting column when the stirring device rotates.