Polymeric carriers for immunohistochemistry and in situ hybridization

摘要

Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.

主权项

1. A conjugate comprising a specific binding moiety, a polymeric carrier, and at least one detectable label, the polymeric carrier having a polymeric portion comprising at least one type of repeating monomeric unit, the polymeric portion further comprising a plurality of reactive functional groups, wherein the specific binding entity is coupled to the polymeric carrier through at least one of the plurality of reactive functional groups, and wherein the at least one detectable label is indirectly coupled to the polymeric carrier through at least another of the plurality of reactive functional groups, wherein the indirect coupling of the at least one detectable label is through a linker.