Neural stem cell having increased passage ability, method for manufacturing neural stem cell having said increased passage abiility, and method for culturing neural stem cells for increasing passage ability of neural stem cells

摘要

The present invention provides a neural stem cell having increased passage ability and a method for manufacturing a neural stem cell having increased passage ability. A neural stem cell in which the N-type calcium channel gene is knocked out or the influx of Ca2+ via the N-type calcium channel is substantially absent can be passaged for at least 4 generations and maintains the differentiation potential into a nerve cell even after passage for 4 generations.

主权项

1. A neural stem cell having the following characteristics:
(a) function or expression of the N-type calcium channel is knocked out in the cell, (b) influx of Ca2+ via the N-type calcium channel is substantially absent when function or expression of the N-type calcium channel is knocked out in the cell, (c) the cell can be passaged for at least 4 generations, and (d) the cell maintains differentiation potential into a nerve cell after passage for 4 generations.