| 主权项 |
1. A chemiluminescent acridinium compound having the following structure: wherein, R1 is a C1-35 alkyl, alkenyl, alkynyl or aralkyl group, each of which may contain up to 20 heteroatoms, or a sulfopropyl or sulfobutyl group, or R1 is a group —Ra—Z; R2 and R3 are independently selected from (i) hydrogen, (ii) an electron donating group, or (iii) a group —Z; Y and Y′ are independently selected from R, —Ra—Z, or —Ra-L; where L is a derivitizable functional group comprising a leaving group, electrophilic group, or nucleophilic group for forming a conjugate with an analyte, analyte analog, or binding partner for an analyte; Z is a zwitterionic group of the form: X is independently selected at each occurrence from a bond, —CH2—, oxygen, sulfur, —NRN—, amide (—NRN(CO)—), carbamate (—NRNC(O)O—), or urea (—NRNC(O)NRN—); R′ and R″ are independently selected at each occurrence from C1-35 alkyl, alkenyl, alkynyl, aryl, or aralkyl, each containing up to 20 heteroatoms; Z1 is a group —Rb—Z2 where Z2 is selected from carboxylate (—COO−), sulfonate (—SO3−), sulfate (—OSO3−), phosphate (—OP(O)(OR)(O−)), or oxide (—O−); Rb is (CH2)m wherein m is an integer from 1 to 4 and in case where Z2 is an oxide (—O−), Rb is absent; n is, independently selected at each occurrence, an integer between one and 12; and R is independently selected at each occurrence from C1-35 alkyl, alkenyl, alkynyl, aryl, or aralkyl groups each containing up to 20 heteroatoms; RN is independently selected at each occurrence from hydrogen, C1-35 alkyl, alkenyl, alkynyl, aryl, or aralkyl groups each containing up to 20 heteroatoms; and Ra is a divalent radical selected from C1-35 alkyl, alkenyl, alkynyl, aryl, or aralkyl group, each optionally containing up to 20 heteroatoms; with the proviso that at least one of R1, R2, R3, Y and Y′ comprises said zwitterionic group Z. |
