| 摘要 |
The present invention pertains to the field of amylases, particularly alpha-amylases, and compositions comprising such amylases. Also described are means and methods for producing such amylases, and uses of amylases and amylase-comprising compositions, for example for cleaning starch-containing stains, textile desizing, starch liquefaction and saccharification, baking and brewing. The invention also discloses means for increasing amylase purity during fermentation, formulation and storage of amylase or amylase-containing formulations. |
| 主权项 |
1. An amylase having a sequence identity of at least 65% to SEQ ID NO. 1 and differing therefrom by two or more substitutions, independently chosen from the following groups:
i) positions in the numbering according to SEQ ID NO. 1: 3, 475, 314, 6, 95, 195, 423, 150, 226, 255, 418, 22, 106, 285, 484, ii) 4, 476, 315, 7, 96, 196, 424, 151, 227, 256, 419, 23, 107, 286, 485, wherein each substitution of group i) is independently selected from any of A, D, E, F, G, H, I, K, L, P, R, S, T, V, W, Y, and wherein each substitution of group ii) is independently selected from any of F, Y, L, I, W, R, E, M. |
