| 摘要 |
Described herein are compounds, methods, and kits for short- and long-term tracking of cell proliferation, differentiation, and/or function. The compounds disclosed herein are rhodamine-based cell-tracking reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells. |
| 主权项 |
1. A method for tracking cell proliferation, differentiation, and/or function, the method comprising the steps of:
a) incubating a mixture of cells and a compound of structural formula (I): or a pharmaceutically acceptable salt thereof, whereinR2, R3, R4, R5 and R6 are each independently H, halogen, alkyl, substituted alkyl, carboxyl, alkylthio, substituted alkylthio, sulfo,-L-Rx, or -L-Sc;R7, R8, R9, R10, R11, R12, R13, R14, R15 and R16 are each independently H, alkyl, substituted alkyl, sulfo, sulfoalkyl, -L-Rx, or -L-Sc; orR7 taken together with R12 are part of an optionally substituted 5- or 6-membered ring;R8 taken together with R13 are part of an optionally substituted 5- or 6-membered ring;R9 taken together with R11 are part of an optionally substituted 5- or 6-membered ring;R10 taken together with R16 are part of an optionally substituted 5- or 6-membered ring;R7 taken together with R8 are part of an optionally substituted 4-, 5-, 6-, or 7-membered ring;R9 taken together with R10 are part of an optionally substituted 4-, 5-, 6-, or 7-membered ring;L is a linker;Rx is a reactive group; andSc is a conjugated substance; b) providing a stimulus to the mixture to elicit a fluorescent signal; and c) analyzing the stimulated mixture. |
