A NOVEL AFFINITY MATRIX AND DEVICES FOR ISOLATION AND PURIFICATION OF RNA AND DNA FOR POINT OF CARE MOLECULAR DEVICES

摘要

The present disclosure relates to nucleic acid extraction and purification methods and devices to accomplish the same. The present disclosure proposes a novel approach to this problem wherein cell isolation and nucleic acid purification can be integrated in a single “step,” by using the same solid phase for both cell adsorption and nucleic acid purification. This is achieved by binding the cells to a solid support as a first step. The same solid support is then used under conditions that lyse the bound cells, and then subsequently enable the nucleic acid to bind to the support. Methods of the present disclosure relate to the isolation of nucleic acid, and especially to a method for isolating DNA from cells, biological or environmental samples using antibiotics, which bind nucleic acids.

主权项

1. A device for isolating nucleic acid from a sample, the device comprising:
a first solid support in fluid communication with a second solid support, said first solid support comprising at least one reagent and said second solid support comprising at least one nucleic acid binding moiety; optionally a sink adjacent to said second solid support; and a third solid support, wherein said third solid support is in removable fluid communication with said second support.