ASX-SPECIFIC PROTEIN LIGASE

摘要

The present invention is directed to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. Further encompassed are methods and uses of these enzymes.

主权项

1. Isolated polypeptide having protein ligase, preferably cyclase, activity comprising or consisting of
(i) the amino acid sequence as set forth in SEQ ID NO:1; (ii) an amino acid sequence that shares at least 60, preferably at least 70, more preferably at least 80, most preferably at least 90% sequence identity with the amino acid sequence set forth in SEQ ID NO:1 over its entire length; (iii) an amino acid sequence that shares at least 80, preferably at least 90, more preferably at least 95% sequence homology with the amino acid sequence set forth in SEQ ID NO:1 over its entire length; or (iv) a fragment of any one of (i)-(iii).