SIRNA IN TANDEM EXPRESSION AND USES THEREOF IN TREATING CHRONIC LYMPHOCYTIC LEUKEMIA

摘要

Provided is an siRNA in tandem expression and uses thereof in treating chronic lymphocytic leukemia, and particularly, provided are a method of a tandem expression for siRNA of BTK, and an siRNA in tandem expression and uses thereof in treating chronic lymphocytic leukemia.

主权项

1. A recombinant nucleotide sequence for inhibiting Bruton's agammaglobulinemia tyrosine kinase (BTK), wherein the nucleotide sequence has a structure of Formula V:
A-(B-L-)p-Z  (V) wherein, A represents an optional sequence at 5′ end (preferably, the length thereof is 0-50 bp, more preferably, 0-20 bp, and most preferably, 0-10 bp); B represents a same or different siRNA sequence that specifically inhibits BTK, or a precursor RNA sequence used to produce the siRNA sequence; L represents an optional interval sequence (preferably, the length thereof is 0-50 bp, more preferably, 0-20 bp, and most preferably, 0-10 bp); p is a positive integer of 1, 2, 3, 4 or 5; Z represents an optional sequence at 3′ end (preferably, the length thereof is 0-50 bp, more preferably, 0-20 bp, and most preferably, 0-10 bp).