| 发明名称 |
RNA-Guided Human Genome Engineering |
| 摘要 |
A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner. |
| 申请公布号 |
US2017044569(A9) |
申请公布日期 |
2017.02.16 |
| 申请号 |
US201514681510 |
申请日期 |
2015.04.08 |
| 申请人 |
President and Fellows of Harvard College |
发明人 |
Church George M.;Mali Prashant;Yang Luhan |
| 分类号 |
C12N15/85 |
主分类号 |
C12N15/85 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of altering a eukaryotic cell comprising
providing to the eukaryotic cell a guide RNA sequence complementary to a target nucleic acid sequence of genomic DNA of the eukaryotic cell, and providing to the eukaryotic cell a Cas 9 protein that interacts with the guide RNA and cleaves the genomic DNA in a site specific manner. |
| 地址 |
Cambridge MA US |
