发明名称 |
Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use |
摘要 |
Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site. |
申请公布号 |
AU2015315651(A1) |
申请公布日期 |
2017.02.16 |
申请号 |
AU20150315651 |
申请日期 |
2015.08.31 |
申请人 |
E. I. du Pont de Nemours and Company;Pioneer Hi-Bred International, Inc. |
发明人 |
Cigan, Andrew Mark;Gao, Huirong;Liu, Zhan-Bin;Mutti, Jasdeep;Podlich, Dean;Scelonge, Christopher |
分类号 |
C12N15/82;A01H5/10 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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