主权项 |
1. A method for providing a reaction mixture of a liquid to be analyzed and at least one reagent liquid in a reaction vessel, in which a pipetting device, which is fastened on an automatically movable or pivotable transfer arm, comprising a heatable pipetting needle is used, and wherein the pipetting needle has a first temperature sensor at a small distance from the tip of the pipetting needle and a second temperature sensor at a greater distance from the tip of the pipetting needle, the method comprising:
aspirating a volume of a reagent liquid into the heated pipetting needle; moving the pipetting needle to a desired location for dispensing the aspirated volume of the reagent liquid into a reaction vessel; then measuring a temperature using the first temperature sensor and a temperature using the second temperature sensor; comparing the temperature measured by the first temperature sensor to a predefined minimum temperature and a predefined maximum temperature; and establishing that the temperature measured by the first temperature sensor either:
exceeds the predefined maximum temperature, ordoes not exceed the predefined maximum temperature and does not fall below the predefined minimum temperature, orfalls below the predefined minimum temperature, wherein if it is established that the temperature measured by the first temperature sensor exceeds the predefined maximum temperature or falls below the predefined minimum temperature, the aspirated volume of the reagent liquid is dispensed from the pipetting needle and then disposed of. |