发明名称 |
RECOMBINANT DEAMIDATED GLIADIN ANTIGEN |
摘要 |
The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. |
申请公布号 |
EP2788760(B1) |
申请公布日期 |
2017.02.15 |
申请号 |
EP20120855209 |
申请日期 |
2012.12.03 |
申请人 |
Bio-rad Laboratories, Inc. |
发明人 |
WALKER, Roger;LU, Yabin;DESAI, Urvee;SHAN, Daming |
分类号 |
G01N33/53;C07K14/415;C12N9/10;C12N15/54;C12N15/62;G01N33/564;G01N33/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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