In vitro assay for predicting renal proximal tubular cell toxicity

摘要

There is provided an in vitro assay for screening a test compound for toxicity in renal proximal tubular cells. The method comprises contacting a test compound with a test population of renal proximal tubular cells; and examining one or more cell morphology features, examining one or more cytoskeleton features, and/or determining cell numbers of the renal proximal tubular cells in the test population and comparing such cell morphology, arrangement of cytoskeletal components and/or cell count with the respective features of a control population. A change in one or more cell morphology features, a change in arrangement of one or more cytoskeleton features or a decrease in cell numbers of the test population relative to the control population is indicative that the test compound is toxic for renal proximal tubular cells.

主权项

1. An in vitro method of screening for renal proximal tubular toxicity of a compound, the method comprising:
contacting a test compound for which renal proximal tubular cell toxicity is unknown with a test population of renal proximal tubular cells grown attached to a support; measuring one or both of the following features of the renal proximal tubular cells in the test population: cell area, and area of nucleus: area of whole cell ratio; comparing the one or more features of the test population with the corresponding one or more features of a control population that has not been contacted with the test compound; and identifying the test compound as toxic for renal proximal tubular cells based on one or both of a decrease in cell area, and an increase in area of nucleus: area of whole cell ratio of the test population relative to the control population.