Inhibitors of micro-RNAs for use for preventing and/or attenuating skin ageing

摘要

Identification and use of compounds which inhibit the expression or activity of micro-RNAs for preventing and/or attenuating ageing. An in vitro method for screening for candidate compounds for preventing and/or attenuating ageing of the skin including (a) bringing at least one test compound in contact with a sample of fibroblasts, (b) measuring the expression or the activity of at least one microRNA chosen from miR-134 and miR-152 in said fibroblasts, and (c) selecting the compounds for which an inhibition of at least 20%, preferably at least 30%, preferably at least 40% of the expression or an inhibition of at least 20%, preferably at least 30%, preferably at least 40% of the activity of at least one microRNA is measured in the fibroblasts treated in (a) compared with the untreated fibroblasts.

主权项

1. An in vitro method for screening for candidate compounds for attenuating ageing of the skin, comprising the following steps:
(a) bringing at least one test compound in contact with at least one sample of fibroblasts; (b) measuring the expression or the activity of at least one microRNA chosen from miR-134 and miR-152 in said fibroblasts; (c) selecting the compounds for which an inhibition of at least 20% of the expression, or an inhibition of at least 20% of the activity, of said at least one microRNA is measured in the fibroblasts treated in step (a) compared with untreated fibroblasts.