Detection of foaming and bulking bacteria in wastewater

摘要

The present invention is directed to the detection of problematic foaming and bulking bacterial species in the biological wastewater treatment process. The invention provides various compositions of matter and methods for the detection of foaming and bulking bacterial species and genera in wastewater and other samples. PCR primers capable of amplifying 16s rRNA gene sequences from various foaming and bulking bacterial species are provided, as are probes that will specifically hybridize with PCR amplification products produced by the disclosed primers. In certain embodiments, the use of the disclosed PCR primers and probes in detection assays is disclosed.

主权项

1. A method of identifying the presence of at least one species of the genus Thiothrix in a sample, comprising the following steps:
isolating bacterial DNA from the sample; performing a PCR amplification reaction which includes the isolated bacterial DNA and PCR primers comprising SEQ ID 5 and SEQ ID 6; collecting the amplification product from the PCR amplification reaction and incubating it, under hybridizing conditions, with one or more Thiothrix identification probes; and assaying for the hybridization of the one or more Thiothrix identification probes to the amplification product, wherein the detectable hybridization of a Thiothrix identification probe to the amplification product is indicative of at least one species of the genus Thiothrix being present in the sample.