Hepatitis B virus mutant, mutant amplification kit and use thereof

摘要

The present invention relates to the field of biotechnology, and discloses a hepatitis B virus mutant, a mutant amplification kit and use thereof. The HBV mutant of the invention takes place mutation of the hepatitis B virus mutant in genome sequence No. 216 and/or 285, the mutation occurred at position 216 is that base T mutates to C, the mutation occurred at position 285 is that base G mutates to A. The two mutations are closely related to the inflammation aggravation of the hepatitis B. The invention further provides a HBV mutant amplification kit to detect the above mutation, the usage of the kit and its application, therefore offering help for the clinical early diagnosis and intervention of severe hepatitis B, and providing references for further studying the functional changes caused by HBV gene mutation.

主权项

1. A kit for identifying hepatitis B (HBV) genetic mutants, wherein the kit comprises:
a) two primers, wherein the two primers consist of the sequences of SEQ ID NO:8 and SEQ ID NO:9, b) dNTP, c) PCR buffer, d) DNA polymerization enzyme, and e) ddH20, wherein the kit provides for the identification of mutations in the HBV genome at positions 216 and/or 285 relative to SEQ ID NO:1, SEQ ID NO:10, or SEQ ID NO:11.