发明名称 Mutant xylanase, manufacturing method and use therefor, and method for manufacturing saccharified lignocellulose
摘要 What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50° C. for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue.
申请公布号 US9567617(B2) 申请公布日期 2017.02.14
申请号 US201615042395 申请日期 2016.02.12
申请人 MITSUI CHEMICALS, INC.;MEIJI SEIKA PHARMA CO., LTD. 发明人 Yanai Hisaaki;Tamai Hiroki;Osabe Masami;Yokoyama Fumikazu;Okakura Kaoru;Inoue Atsushi
分类号 C12N9/24;C12N9/42;C07H21/04;C12N15/64;C12P19/02;C12P19/14;C11D3/386;A21D8/04;C13K1/02;C13K13/00 主分类号 C12N9/24
代理机构 Foley & Lardner LLP 代理人 Foley & Lardner LLP
主权项 1. A nucleic acid encoding a mutant xylanase, the mutant xylanase comprising the following amino acid residues substitutions in the amino acid sequence of SEQ ID NO: 1: a leucine residue substituted for an asparagine residue at position 29; an arginine residue substituted for a lysine residue at position 58; an amino acid residue, other than a tyrosine residue, substituted for a tyrosine residue at position 27; and an amino acid residue, other than an asparagine residue, substituted for an asparagine residue at position 44, or the mutant xylanase comprising 1 to 10 amino acid residues substitution in the amino acid sequence of SEQ ID NO: 1 in addition of the following amino acid residues substitutions: a leucine residue substituted for an asparagine residue at position 29, an arginine residue substituted for a lysine residue at position 58, a phenylalanine residue substituted for a tyrosine residue at position 27, and a serine residue substituted for an asparagine residue at position 44.
地址 Tokyo JP