| 摘要 |
The present invention provides a method for constructing a variable region sequencing library, and a method for determining a variable region nucleic acid sequence. The method for constructing the variable region sequencing library comprises: adopting a sample containing nucleic acid encoding the variable region as a template, amplifying the nucleic acid encoding the variable region so as to obtain a first amplification product, and adding a sequencing linker to at least one of both terminals of the first amplification product so as to obtain the variable region sequencing library, wherein the primers used by the amplifying comprise: a first primer comprising at least a nucleic acid molecule specifically recognizing the framework encoding sequence of the V region, and a second primer comprising at least a nucleic acid molecule specifically recognizing the encoding sequence of the J region or C region. With the method of the present invention, the variable region sequencing library can be effectively constructed. |
