ELISA for a Naturally-Occurring Soluble Truncated Form of IL-23 Receptor

摘要

A naturally-occurring soluble truncated IL-23Rα protein (i.e., Δ9 IL-23Rα) is shown to be present in a biological sample and can serve as a diagnostic tool for autoimmune diseases. There is provided an enzyme-linked immunosorbent assay (ELISA) and test kit for the serological detection of the soluble truncated form of IL-23Rα protein. More particularly, antibody-sandwich ELISA method and kits for Δ9 IL-23Rα as an antigen were developed to detect Δ9 IL-23Rα levels in biological samples from a mammal and a human patient and are used as a diagnostic index. The present disclosed ELISA has utility as a diagnostic tool to detect Crohn's disease in patients using EDTA-plasma.

主权项

1. A kit for detecting a Δ9 isoform of IL-23Rα, comprising: a) a first antibody that binds to carboxyl-terminal truncated Δ9 isoform of IL-23Rα, wherein the carboxyl-terminal truncated Δ9 isoform of IL-23Rα has the amino acids 318-348 of IL-23Rα; and; b) instructions for using the antibody for detecting Δ9 isoform of IL-23Rα, wherein said Δ9 isoform has an amino acid sequence of SEQ ID NO: 2.