METHOD AND KIT FOR THE DETECTION OF MICROORGANISMS

摘要

There is provided a method of detecting an analyte in a sample. The method is based on colorimetry and also on the binding affinity between the analyte and a chemical substrate which may be a recognition receptor thereof. The method involves a support and a colored carrier. A kit for use in the detection is also provided.

主权项

1. A method of detecting an analyte in a sample, comprising:
(a) providing a support of a first color; (b) attaching on a surface of the support, a chemical substrate having a binding affinity with the analyte to be detected, to obtain a functionalized support; (c) contacting the functionalized support with the sample; (d) providing a carrier of a second color different from the first color and capable of masking the first color; (e) attaching on a surface of the carrier, the chemical substrate having a binding affinity with the analyte, to obtain a functionalized carrier; (f) contacting the functionalized support obtained at step (c) with the functionalized carrier, such that a visual inspection of the support reveals only the second color; and (g) submitting the support obtained at step (f) to a step aimed at removing the carrier from the support,wherein persistence of the second color is indicative of the presence of the analyte in the sample, and disappearance of the second color is indicative of the absence of the analyte.