| 发明名称 |
REGULATABLE PROMOTER |
| 摘要 |
A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate or at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system. |
| 申请公布号 |
US2017037418(A1) |
申请公布日期 |
2017.02.09 |
| 申请号 |
US201615335132 |
申请日期 |
2016.10.26 |
| 申请人 |
LONZA LTD. |
发明人 |
MATTANOVICH DIETHARD;GASSER BRIGITTE;MAURER MICHAEL;PRIELHOFER ROLAND;KLEIN JOACHIM;WENGER JANA |
| 分类号 |
C12N15/81;C12N9/02;C07K16/00;C12N15/67;C07K14/39;C12N15/63;C12P21/00 |
主分类号 |
C12N15/81 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps
a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with no or a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter of the cell, and c) producing and recovering the POI. |
| 地址 |
VISP CH |
