RECOMBINANT FIBRINOGEN

摘要

The present invention relates to nucleotide sequences encoding a fibrinogen alpha, beta or gamma chain. The sequences are optimized for expression in a eukaryotic cell culture system. Such optimized nucleotide sequences allow for the efficient expression of recombinant fibrinogen and variants thereof in intact form in a eukaryotic cell culture system.

主权项

1. A nucleotide sequence which is optimized for expression in a mammalian cell culture system, comprising:
(i) a nucleotide sequence according to SEQ ID NO. 4 or 7, or a part thereof which comprises nucleotides 60 to 1932 of SEQ ID NO. 4, nucleotides 60 to 1887 of SEQ ID NO. 4 or nucleotides 60-2598 of SEQ ID NO. 7, or a nucleotide sequence which has a sequence which is at least 90% identical to SEQ ID NO. 4 or 7 and which encodes a fibrinogen alpha chain, or (ii) a nucleotide sequence according to SEQ ID NO. 5, or the part thereof which comprises nucleotides 93 to 1473 of SEQ ID NO. 5, or a nucleotide sequence which has a sequence which is at least 90% identical to SEQ ID NO. 5 and which encodes a fibrinogen beta chain or (iii) a nucleotide sequence according to SEQ ID NO. 6 or 12, or a part thereof which comprises nucleotides 81 to 1311 of SEQ ID NO. 6 or nucleotides 81 to 1359 of SEQ ID NO. 12, or a nucleotide sequence which has a sequence which is at least 90% identical to SEQ ID NO. 6 or 12 and which encodes a fibrinogen gamma chain.