| 主权项 |
1. A method of binding a zinc finger protein to a target site in an Htt gene, the target site comprising the sequence CAGCAG, CTGCTG, GCAGCA, GCTGCT, AGC, CAG, CTG, GCA, GCT or AAG in a cell, the method comprising providing to the cell a non-naturally occurring zinc finger protein comprising 4, 5 or 6 zinc finger domains ordered F1 to F4, F1 to F5 or F1 to F6, respectively, wherein the zinc finger protein binds to the Htt gene and comprises one- or two-finger domains at positions F2 or F2 and F3, the two-finger domains consisting of the recognition helix regions sequences in F2 and F3 as shown in a single row of Table 2C and further wherein:
(i) F2 and F3 of the zinc finger protein comprise the recognition helix regions shown in SEQ ID NO:161, 162, 163, 164, 165, 166 or 167 to bind to the target sequence CAGCAG; (ii) F2 and F3 of the zinc finger protein comprise the recognition helix regions shown in SEQ ID NO: 168 to bind to the target sequence CTGCTG; (iii) F2 and F3 of the zinc finger protein comprise the recognition helix regions shown in SEQ ID NO:169, 170, 171 and 172 to bind to the target sequence GCAGCA; (iv) F2 and F3 of the zinc finger protein comprise the recognition helix regions shown in SEQ ID NO: 173 and 174 to bind to the target sequence GCTGCT; (v) F2 comprises the recognition helix region shown in SEQ ID NO:175 to bind to the target sequence AGC; (vi) F2 comprises the recognition helix region shown in SEQ ID NO: 176 or 177 to bind to the target sequence CAG; (vii) F2 comprises the recognition helix region shown in SEQ ID NO:42 or 148 to bind to the target sequence CTG; (viii) F2 comprises the recognition helix region shown in SEQ ID NO:6, 18 or 178 to bind to the target sequence GCA; (ix) F2 comprises the recognition helix region shown in SEQ ID NO:31 to bind to the target sequence GCT; and (x) F2 comprises the recognition helix region shown in SEQ ID NO:65 to bind to the target sequence AAG. |
