发明名称 |
METHOD FOR PREPARING CARBOHYDRATE CONVERTING ENZYME LIBRARY USING DOMAIN SWAPPING BASED ON AMINO ACID CONSERVED SEQUENCE AND USE THEREOF |
摘要 |
The present invention relates to a method for preparing a chimeric carbohydrate converting enzyme library, the chimeric carbohydrate converting enzyme library prepared through the method and the carbohydrate converting enzyme prepared through the library, wherein the method comprises identifying a conserved sequence of a carbohydrate converting enzyme and recombining the conserved sequence. By using the smart carbohydrate converting enzyme library according to the present invention, the effect can be realized in that a carbohydrate converting enzyme can be obtained in which the activity and function of the enzyme is not lost at an efficiency of 40% or more, unlike a typical library, and a carbohydrate converting enzyme having various physical and biochemical properties is obtainable. Therefore, an enzyme suitable for various conditions can be selected and used. |
申请公布号 |
WO2017023071(A1) |
申请公布日期 |
2017.02.09 |
申请号 |
WO2016KR08463 |
申请日期 |
2016.08.01 |
申请人 |
KYUNGPOOK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION |
发明人 |
LEE, Dong Woo;SHIN, Sun Mi;LEE, Yong Jik;LEE, Sang Jae |
分类号 |
G01N33/573;C40B40/10;C40B50/06 |
主分类号 |
G01N33/573 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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