发明名称 |
Method for Gene Amplification |
摘要 |
The present invention provides a double-stranded DNA constructed specifically for high speed gene amplification, a method for gene amplification and a method for synthesizing protein. The gene amplification system of the present invention used a site-specific recombinase such as Cre-lox system and target sequence thereof to efficiently induce a type of replication referred to as a double rolling-circle replication (DRCR). Amplification unit, whose structure is shown in FIG. 2 (a), is constructed in animal and other cells. DRCR is induced by two recombination events triggered by a site-specific recombinase (Cre) when each replication folk progresses between each pair of target sequences (lox sequences). |
申请公布号 |
US2017037428(A1) |
申请公布日期 |
2017.02.09 |
申请号 |
US201615261274 |
申请日期 |
2016.09.09 |
申请人 |
Genodive Pharma Inc. |
发明人 |
Horiuchi Takashi;Watanabe Takaaki |
分类号 |
C12N15/85 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
1. A double-stranded DNA represented by a-b-c-d or a-c-b-d, wherein one of a and b is a double-stranded DNA fragment comprising a first target sequence of a site-specific recombinase, and the other is a double-stranded DNA fragment comprising an inverted sequence of said first target sequence; and one of c and d is a double-stranded DNA fragment comprising a second target sequence of the site-specific recombinase and the other is a double-stranded DNA fragment comprising an inverted sequence of said second target sequence; a replication origin and at least one target gene to be amplified are inserted anywhere between a and d; and arbitrary DNA sequences may be inserted among above fragments. |
地址 |
Kanagawa JP |