METHOD OF OBTAINING A STRAIN OF BACTERIOFAGE, SPECIFIC STRAINS OF BACTERIOPHAGE AND USE THEREOF

摘要

A method for obtaining a strain of bacteriophage specific to a selected strain of bacteria was found as well as bacteriophage strains obtained in this way. Moreover, application of bacteriophages in manufacturing of the preparation for preventing and fighting infections of farm animals, especially poultry, with pathogenic strains of bacteria sensitive to these phages was described.

主权项

1. The method of obtaining a strain of bacteriophage specific to a selected strain of bacteria, characterized in that:
a) a collection of bacteriophage strains containing a strain of bacteriophage specific to a selected strain of bacteria is obtained, b) culture of a selected strain of bacteria on a sterile culture medium is conducted, c) the culture samples are applied on the special multi-well measuring plate, then a suspension of tested bacteriophage strain at various concentrations is added and it is incubated at 37° C. for at least 4 hours, d) resazurin is added to the culture samples and incubation is continued in the dark at about 37° C. for at least 3 hours, e) the colour or fluorescence of culture is being controlled, as well as bacteripohage strain contained in the culture that retains the blue colour or shows no significant increase in fluorescence compared with the control sample, is identified as a strain of bacteriophage specific to a selected strain of bacteria. A sterile sample that was treated with the same incubation is used as a control sample. f) identified strain of bacteriophage specific to a selected strain of bacteria is propagated.