Lyophilized Lentiviral Vector Particles, Compositions and Methods

摘要

Methods of making lyophilized lentiviral vector particles are provided. Compositions comprising lyophilized lentiviral vector particles are also provided. Methods of administering a lentiviral vector particle to a subject and uses of lentiviral vector particle compositions are also provided.

主权项

1. A method for making lyophilized lentiviral vector particles, the method comprising:
a) providing a cell supernatant comprising lentiviral vector particles, b) purifying the lentiviral vector particles in the supernatant, c) concentrating the purified lentiviral vector particles, d) freezing the concentrated lentiviral vector particles to provide frozen lentiviral vector particles, e) lyophilizing the frozen lentiviral vector particles to provide lyophilized lentiviral vector particles, and f) storing the lyophilized lentiviral vector particles for at least three days.