ISOLATION OF NUCLEIC ACID

摘要

A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described.

主权项

1. A method for isolating a nucleic acid, which comprises:
(i) binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer; (ii) washing the bound nucleic acid with a wash solution; and (iii) eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH;wherein the wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution; and wherein the pH of the wash solution is pH 6.0 or less, and wherein the nucleic acid is eluted from the solid phase at a temperature above ambient temperature.