NOVEL ASSAY FOR EARLY DETECTION OF A DISEASE USING A MAGNETIC NANOPARTICLE BIOSENSOR

摘要

A multiplex hand-held diagnostic biosensor, using two inflammatory salivary biomarkers, Human Neutrophil Elastase (HNE) and Cathepsin-G, was constructed made to potentially detect Periodontitis at an early stage is described. The use of magnetic nanoparticle biosensor method used as a device was based on the measurement of proteolytic activity using specific proteases probes. The magnetic nanoparticle biosensor device is capable of specific and quantitative detection of HNE and Cathepsin-G in solution and in spiked saliva samples with a lower detection limit of 1 pg/mL and 100 fg/mL for HNE and Cathepsin-G, respectively.

主权项

1. A method to detect a disease having a marker enzyme, comprising:
collecting a biological sample using a support tool embedded with a magnetic nanoparticle biosensor; attaching a marker peptide coupled carboxy terminated magnetic bead to a gold sensor support using a specific method; reacting the biological sample and the marker peptide coupled carboxy terminated magnetic bead to form a conjugate; and measuring to detect an association or dissociation of the conjugate on the gold sensor support for presence and intensity of the disease, effect of treatment for the disease and absence of the disease in a mammal.