Systems And Methods Enabling Patch-Clamp Re-Use

摘要

A method for cleaning patch-clamp glass pipette electrodes that enables their re-use. By immersing pipette tips or planar patch clamp chips into a detergent, followed by rinsing, pipettes and planar patch clamp chips were re-usable at least ten times with little to no degradation in signal fidelity, in experimental preparations ranging from human embryonic kidney cells to neurons in culture, slices, and in vivo.

主权项

1. A method of detecting one or more characteristics of cells comprising:
forming a resistance seal with a membrane of a cell with an apertured surface; forming a resistance seal with a membrane of a different cell with the apertured surface; and preparing the apertured surface; wherein preparing the apertured surface enables re-use of at least portions of the same apertured surface with different cells.