| 摘要 |
The invention relates to a method for detecting at least one nucleic acid to be detected (10) in a solution, comprising the steps of: providing at least one dye (8), wherein the solution and/or a reaction vessel (R), wherein the solution is present, comprises the dye (8) and wherein the at least one dye (8) is adapted to emit emission light due to an optical excitation by excitation light; providing at least one absorber (1) in the solution, wherein the absorber (1) is adapted to cause an attenuation of the emission light and/or the excitation light and wherein the attenuation is influenced by a bonding of the at least one absorber (1) to the nucleic acid to be detected (10); and radiating excitation light into the solution and measuring an intensity of the emission light; wherein the attenuation of the emission light and/or the excitation light by the at least one absorber occurs independently of a quantum yield of the at least one dye. Furthermore, the present invention relates to a corresponding kit, comprising at least one corresponding dye and at least one corresponding absorber. |
