GENETICALLY MODIFIED IPS CELLS THAT CARRY A MARKER TO REPORT EXPRESSION OF NEUROGENIN3, TPH2, FOXO1 AND/OR INSULIN GENES

摘要

Provided herein are insulin-negative cells that have been genetically modified to report expression of one or more target genes. Exemplified are reporter cell lines that provide a readout of Ngn3, Foxo1 or Tph2 expression. Reporter cells are used to screen for agents that affect expression of one or more of these genes to identify agents capable of converting gut progenitor cells to insulin-positive cells.