发明名称 |
REAGENT FOR ENDOTOXIN ASSAY AND METHOD FOR ENDOTOXIN ASSAY USING THE SAME |
摘要 |
This invention provides (1) a reagent for endotoxin assay which comprises aprotinin and a limulus amebocyte lysate reagent, (2) a kit for endotoxin assay which comprises the limulus amebocyte lysate reagent and a reagent containing aprotinin, (3) a method for assaying endotoxin in a sample using the limulus amebocyte lysate reagent in which aprotinin is added to the lysate reagent and/or the sample, (4) a method for assaying endotoxin in a serine protease-containing sample using the limulus amebocyte lysate reagent in which the sample is allowed to contact with an aprotininimmobilized insoluble carrier in advance of endotoxin assay, (5) a carrier for pretreating a serine protease-containing sample on which aprotinin is immobilized, (6) a method for inhibiting factor G activation in which aprotinin is added to the limulus amebocyte lysate reagent and (7) a factor G activation inhibitor which comprises aprotinin as an active ingredient. Endotoxin assay can be effected based on the factor C system reaction, without influences of factor G contained in the limulus amebocyte lysate reagent and/or serine proteases contained in samples.
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申请公布号 |
CA2116315(A1) |
申请公布日期 |
1994.08.27 |
申请号 |
CA19942116315 |
申请日期 |
1994.02.23 |
申请人 |
SEIKAGAKU KOGYO KABUSHIKI KAISHA |
发明人 |
TANAKA, SHIGENORI;TAMURA, HIROSHI;AITA, KAZUHIRO |
分类号 |
G01N33/543;G01N33/579;(IPC1-7):G01N33/579;C12Q1/37 |
主分类号 |
G01N33/543 |
代理机构 |
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主权项 |
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地址 |
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