发明名称 |
Microfluidic device for real-time clinical monitoring and quantitative assessment of whole blood coagulation |
摘要 |
In accord with one aspect, a microfluidic coagulation assessment device defining a plurality of microchannels is provided, wherein a blood sample is driven through the microchannels at a substantially constant flow rate and a controller is configured to, in combination with a timer and a pressure sensing device, determine a first pressure value (or flow value) at an initiation of flow, a first time (Tpg) at which a second pressure value is about twice the determined first pressure value, and a second time (Tpf) at which a third pressure value is about (1+e) times the determined first pressure value and establish a subject coagulation model predictive of channel occlusion therefrom. In another aspect, the blood sample is driven through the microchannels at a substantially constant pressure and a controller is configured to, in combination with a timer and a flow sensing device make the determination based on flow rate. |
申请公布号 |
US9562914(B2) |
申请公布日期 |
2017.02.07 |
申请号 |
US201415028667 |
申请日期 |
2014.10.16 |
申请人 |
President and Fellows of Harvard College |
发明人 |
Jain Abhishek;Waterhouse Anna;Super Mike;Ingber Donald E.;Leslie Daniel C. |
分类号 |
G01N33/49;G01N33/86;B01L3/00;G06F19/24 |
主分类号 |
G01N33/49 |
代理机构 |
Nixon Peabody LLP |
代理人 |
Nixon Peabody LLP |
主权项 |
1. A microfluidic coagulation device, comprising:
at least one substrate defining a plurality of microchannels; a first port at a first end portion of the substrate, the first port connecting to first ends of the plurality of microchannels; a second port at a second end portion of the substrate, the second port connecting to second ends of the plurality of microchannels; a first sensing device configured to determine a pressure value in, or relating to, a pressure across the plurality of microchannels; a timer; and a controller configured to determine, in combination with the first sensing device and the timer, a first pressure value at an initiation of flow, a first time at which a second pressure value is determined to be about twice the determined first pressure value, and a second time at which a third pressure value is about (1+e) times the determined first pressure value, and further configured to establish a subject coagulation model predictive of channel occlusion in accord with the relationΔP(t)ΔP(0)=1+ⅇt-TpgTpf-Tpg wherein Tpf is the second time and Tpg is the first time, and wherein a differential pressure or flow rate/shear applied across the first port drives a blood sample across the plurality of microchannels at a substantially constant flow rate. |
地址 |
Cambridge MA US |